BLOCK-iT™ RNAi Designer

The easiest way to design effective RNAi molecules for great results

Use the BLOCK-iT™ RNAi Designer to design and order siRNA, Stealth RNAi™ siRNA, miR RNAi inserts and shRNA inserts for any target.

See also: BLOCK-iT™ RNAi Express. Simplified online ordering of pre-designed and validated Stealth Select RNAi™ siRNA and BLOCK-iT™ miR RNAi Select for human, mouse and rat targets.

New: PlateSelect™: Configure and order 1 nmol scale siRNA and Stealth RNAi™ siRNA in 96-well plates.

Target Design Options:

Stealth RNAi™ siRNA

siRNA

miR RNAi

shRNA

siRNA to Stealth RNAi™ siRNA

siRNA to shRNA

Step 1: Enter an accession number or provide a nucleotide sequence.

Accession number:

Nucleotide sequence: Enter only A, C, G, T, and U. See the online Help for additional information

Step 2: If you entered an accession number in Step 1, select regions for target design

Open reading frame (ORF)

5' UTR

3' UTR

Step 3: Choose database for Blast

NOTE: BLAST is used to compare input sequence with sequences in the database to find unique regions against which to design RNAi targets. The databases contain representative gene sequences for that species. Blast databases were updated on May 01, 2008 and the design output reflects the most up-to-date designs.

Step 4: Choose minimum and maximum G/C percentage

Minimum G/C percentage:

Maximum G/C percentage:

Step 5: Click "RNAi Design" to design your Stealth RNAi™ siRNA molecules.

Guarantee: The BLOCK-iT™ RNAi Designer is such an effective tool for the design of Stealth RNAi™ siRNA if you order the three best Stealth RNAi™ siRNA sequences designed by the BLOCK-iT™ RNAi Designer, we guarantee that two of them will give greater than 70% knockdown of mRNA, given that transfection efficiency in your experiment is at least 80%.

If you already know your RNAi oligo sequence: Order oligos online at Order Custom Primers or by e-mail/Fax.