BLOCK-iT™ RNAi Designer

The easiest way to design effective RNAi molecules for great results

The BLOCK-iT™ RNAi Designer, winner of the 2004 Frost & Sullivan Excellence in Research Award, utilizes a highly effective, proprietary algorithm. The Stealth™ RNAi design algorithm has recently been even more highly refined through bioinformatic analysis of multiple indicators for over one thousand functionally validated Stealth™ RNAi sequences. The BLOCK-iT™ RNAi Designer update demonstrates superior design performance for Stealth™ RNAi design in any organism.

Last updated: November 22, 2005.

Available Now: The BLOCK-iT™ RNAi Express. Easy online ordering of bench-tested Validated Stealth™ RNAi, ready-to-order Stealth™ Select RNAi, and bench-tested BLOCK-iT™ Pol II miR Validated miRNA Vector Duopaks.

PlateSelect™: NEW: Order made-on-demand RNAi in customizable plate format at 1nmole scale!

Target Design Options:

Stealth™ RNAi

Step 1: Enter an accession number or provide a nucleotide sequence.

Accession number:

Nucleotide sequence: Enter only A, C, G, T, and U. See the online Help for additional information

Step 2: If you entered an accession number in Step 1, select regions for target design

Open reading frame (ORF)

5' UTR

3' UTR

Step 3: Choose database for Blast

NOTE: BLAST is used to compare input sequence with sequences in the database to find unique regions against which to design RNAi targets. The databases contain representative gene sequences for that species. Blast databases were updated on May 01, 2008 and the design output reflects the most up-to-date designs.

Step 4: Choose minimum and maximum G/C percentage

Minimum G/C percentage:

Maximum G/C percentage:

Step 5: Click "RNAi Design" to design your Stealth™ RNAi molecules.

Guarantee: The BLOCK-iT™ RNAi Designer is such an effective tool for the design of Stealth™ RNAi that if you order the three best Stealth sequences designed by the BLOCK-iT™ RNAi Designer, we guarantee that two of them will give greater than 70% knockdown of mRNA, given that transfection efficiency in your experiment is at least 80%.

If you already know your RNAi oligo sequence: Order oligos online at Order Custom Primers or by e-mail/Fax.